Methods at Your Bench
Polymerase Chain Reaction
Table of Contents
Cloning with PCR
   Site-directed mutagenesis
using QuikChange™ Stratagene kit (you get required mutation in one day)
Free
mutagenesis. Fast, requires 1-2 days to accomplish insertions or substitutions up to 140
bases long or any deletions
Screening colonies by PCR, after transformation.
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