Methods at Your Bench

Electrophoresis

Table of Contents

Agarose Gels

Agarose Gel Electrophoresis (basic protocol)
Agarose gel electrophoresis for 7x10 cm glass plate.

SDS Gels

SDS discontinuous polyacrylamide gel electrophoresis

SDS tricine discontinuous polyacrylamide gel electrophoresis.Gives better MW range and resolution than glycine SDS method (separates 5-200kDa on one gel), but runs somewhat slower.

An alternative destaining protocol (works faster and does not require dangerous chemicals)

Silver staining of SDS polyacrylamide gels.